Methylene blue USP is used in both science and therapy in a variety of ways. It complies with the requirements stated in the 42nd edition of the United States Pharmacopoeia (USP). Medication quality, purity, strength, and other specifications are governed by the USP, a standard reference for pharmaceuticals. In histology and microbiology labs, methylene blue USP is used as a dye to stain biological materials, enhancing the visibility of cellular features under a microscope. In some medical operations, it can also be employed as an indicator colour. It also has therapeutic uses and is used to treat particular diseases.